Solvent Extraction of Fatty Acids from Amylose Inclusion Complexes

The extractability of palmitic and other common fatty acids from inclusion complexes with potato amylose was studied using a wide range of common lipid solvents. At ambient temperature negligible yields were obtained with light petroleum or diethyl ether, and yields were low with most other solvents except those containing methanol or water. At 100°C, methanol, ethanol and propanol and all alcohol‐water mixtures gave good yields. Complexes of amylose with 1‐ O ‐palmitoyllysophosphatidylcholine also gave good yields when extracted with propanol‐water (3:1) at 100°C. Since more rigorous conditions are required for extraction of lipids from native cereal starch granules, it seems that the barrier to lipid extraction is the structure of the granule rather than the resistance to lipid extraction of any possible inclusion complexes in the granules.