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May 1, 1992Journal of Cellular Physiology

Cytoskeletal events underlying dendrite formation by cultured pigment cells

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Authors

JLJ.‐P. LacourCentre Hospitalier Universitaire de NicePGPhilip GordonBoston UniversityMEMark S. EllerSylvester Comprehensive Cancer Center

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Implication

In vitro experimental study demonstrates distinct roles for actin and microtubules during dendrite outgrowth and maintenance in pigment cells, suggesting melanocytes can model neurite outgrowth.

Key Points

  • To determine the cytoskeletal and protein synthesis requirements underlying dendrite formation and maintenance in cultured melanocytes and melanoma cells.
  • Stimulated human melanocytes and B16 melanoma cells with keratinocyte-conditioned medium (KCM) and quantified dendrite outgrowth using computerized image analysis.
  • Treated cultures before, during, or after KCM stimulation with actin disrupters (cytochalasin B), microtubule-disrupting agents (colchicine, nocodazole, vinblastine), and protein synthesis inhibitors (cycloheximide, actinomycin D).
  • Visualized cytoskeletal reorganization using anti-tubulin monoclonal antibodies and rhodamine-phalloidin while analyzing beta-actin and beta-tubulin mRNA levels.
  • Cytochalasin B completely and reversibly blocked KCM-induced dendrite formation when added prior to or during stimulation, but did not disrupt established dendrites.
  • Microtubule-disrupting agents did not prevent initial dendrite formation, but dismantled pre-existing dendrites.
  • Protein synthesis inhibitors reversibly abolished dendrite outgrowth, whereas cytoskeletal restructuring occurred without alterations in beta-actin or beta-tubulin mRNA expression.

Cite This Study

Lacour et al. (1992) studied this question.

synapsesocial.com/papers/6a85bb78b451cea71dae78cehttps://doi.org/10.1002/jcp.1041510210
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