Cherry leaf roll virus ( CLRV ) isolates from M alus domestica , R ibes rubrum , R ubus idaeus , R umex obtusifolius and V accinium darrowii were characterized based on nucleotide sequences of a 371 bp fragment of the 3′ untranslated region ( UTR ) of their genomic RNA s, symptoms in the herbaceous hosts, C henopodium amaranticolor , C henopodium quinoa , N icotiana benthamiana , N icotiana occidentalis and N icotiana tabacum , and seed transmission in N . occidentalis . The different isolates induced a range of localized and systemic disease symptoms, of varying severity, in the herbaceous hosts. The isolates from M . domestica , R . rubrum , R . obtusifolius and V . darrowii all showed greater than 80% seed transmission in N . occidentalis , but no seed transmission was observed for the R . idaeus isolate. Based on symptoms and seed transmission, the isolates appear to be biologically distinct strains of CLRV . Phylogenetic analysis of the nucleotide sequences from the 3′ UTR , commonly used to detect CLRV , showed that four isolates from M . domestica , R . rubrum , R . idaeus and V . darrowii were almost identical but an isolate from R . obtusifolius exhibited a pairwise nucleotide difference of up to 5·4% when compared to these isolates. There was no obvious correlation between sequence differences and symptomatology.
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Woo et al. (2013) studied this question.