Interleukin 8serumconcentration, but not lactate dehydrogenase activity, positively correlates to CD34 antigen in melanoma tumors

CD34 promotes melanoma cell motility, negative regulation of cellular response to hypoxia and positive regulation of vasculogenesis. Interleukin 8 (IL-8) is responsible for angiogenic response in endothelial cells, increases proliferation, metastasis, and survival of melanoma cells. The aim of our study was evaluation of relationship between CD34 immunoexpression and IL-8 serum concentrations in melanoma patients. The study was conducted on patients with melanoma that were divided in: Clark II (17 patients - 19.3%), Clark III (33 patients - 37.5%), Clark IV (22 patients - 25%), and Clark V (16 patients - 18.2%) levels. CD34 expression was absent for Clark II melanomas, and positive for Clark III (6.1%), Clark IV (40.9%), and Clark V (56.2%). The CD34 immune-mark was highly positive only for Clark IV and V levels. Interleukin 8 (IL-8) had high values (above 15 pg/mL) for all patients with melanoma (58.9% - Clark II; 87.8% - Clark III; 90.9% - Clark IV and 93.7% - Clark V). We have found a strong and statistically significant correlation between CD34 and IL-8 for Clark IV (r = 0.54, P < 0.05) and Clark V (r = 0.72, P < 0.05) melanomas. CD34 and IL-8 are associated with poor prognosis of melanoma, metastasis, and neoangiogenesis.