The effect of inactivation of water‐splitting enzyme on the thermoluminescence of isolated chloroplasts was investigated. The inhibitory treatments used included Tris‐washing, alkaline pH in the presence of the uncoupler gramicidin, incubation with a high concentration of magnesium ions and different chaotropic agents. It was found that inhibition of oxygen evolution resulted in the disappearance of the main thermoluminescence band at +20°C. The A band which appears at — 10°C and has been related to the S 4 state of the water‐splitting enzyme (Inoue, 1981) was not considerably affected by the inhibition of oxygen evolution. The results presented here indicate that the participation of the S 4 state of water‐splitting enzyme in the generation of the A band should be reinvestigated.
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Rózsa et al. (1982) studied this question.
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