Fourier Transform Infrared (FTIR) spectroscopy and powder neutron diffraction (PND) were performed in human subchondral bone covered (C+) or not covered by cartilage (C−) to study hydroxyapatite. With FTIR, the carbonation rate was 30% with identical spectra in C+ and C−. With PND, the width of the diffraction peak ( hkl = 002) highlighted the anisotropy of nanocrystals (with needle and/or platelet-like shape) along the c -axis with average length of 50 nm and thickness of 10 nm and with no difference between C+ and C−.
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Chappard et al. (2016) studied this question.
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