What question did this study set out to answer?

To identify and characterize the biochemical properties of a somatomedin inhibitor present in the serum of fasted rats.

January 1, 1983Endocrinology

Partial Characterization of Somatomedin Inhibitor in Starved Rat Serum*

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Authors

WSW. D. SalmonUniversity of North Carolina at Chapel Hill LHLeslie A. HolladayCase Western Reserve University VBV. JEAN BURKHALTER

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Implication

Experimental study uncovers a heat-stable acidic protein inhibitor of somatomedin in starved rat serum, suggesting a regulatory mechanism for cartilage growth during nutrient deprivation.

Key Points

To identify and characterize the biochemical properties of a somatomedin inhibitor present in the serum of fasted rats.
Harvested serum from pituitary-intact rats fasted for 4 days and fed hypophysectomized control rats.
Assayed inhibitory activity in vitro on hypophysectomized rat cartilage by measuring sulfate incorporation into proteoglycans and thymidine incorporation into DNA.
Evaluated stability under variable thermal and pH conditions, organic extractions, ion exchange chromatography, and gel filtration.
Starved rat serum completely blocked the cartilage-stimulating effects of an equivalent volume of normal rat serum, inhibiting somatomedin activity more potently than insulin-mediated actions.
Serum from fed hypophysectomized rats displayed roughly half the inhibitory potency of starved rat serum, indicating that the pituitary gland is not required for inhibitor production.
The inhibitor remained active after boiling at 100 °C under acidic conditions (pH 2.0–3.5), was extractable into acid-ethanol, and eluted as an acidic protein with an apparent molecular weight of 27,000–40,000 Da.

Cite This Study

Salmon et al. (1983) studied this question.

synapsesocial.com/papers/6a865dddf5121d64a72ed42e https://doi.org/10.1210/endo-112-1-360

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Implication

Experimental study uncovers a heat-stable acidic protein inhibitor of somatomedin in starved rat serum, suggesting a regulatory mechanism for cartilage growth during nutrient deprivation.

Partial Characterization of Somatomedin Inhibitor in Starved Rat Serum*

Discussion

Member takes

Implication

Key Points

Cite This Study

Partial Characterization of Somatomedin Inhibitor in Starved Rat Serum*

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider