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We present a new method that enables the generation of arbitrary positioned dual-beam traps without additional hardware in a single-beam holographic optical tweezers setup. By this approach, stable trapping at medium numerical aperture and long working distance is realized on a standard Zeiss Axiovert 200 M research microscope. Simulations of focus separations and spherical aberrations were performed and first experimental results are presented.
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Zwick et al. (2009) studied this question.
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