Enantiomer separation of DNP‐amino acids by capillary electrophoresis using chiral buffer additives

The direct enantiomeric analysis of six DNP‐amino acids by capillary electrophoresis is reported. This study describes a comparison of several chiral buffer additives. Both the natural proteins BSA, AGP, ovomucoid and casein, and the cyclic oligosaccharides α‐, β‐, and γ‐cyclodextrin were used as chiral selectors. For the first time, casein from goat milk was successfully applied for chiral discrimination. The influence of the modifier n ‐propanol on chiral discrimination is demonstrated with BSA as a buffer additive.