Phylogenetic Diversity Analysis of Archaeal in the Pit Mud with Different Cellar Age

In order to investigate the clusters and succession rules of archaeal in the pit mud,the total DNAs were extracted from the pit mud with cellar age of 20,50 and 150 years,respectively.The community structure was studied by polymerase chain reaction-amplified rDNA restriction analysis(PCR-ARDRA) and sequencing of 16S rDNA.The results showed that 191 clones from the 16S rDNA library fell into 22 operational taxonomic units(OTUs) from 6 groups including Methanosarcina,Methanobacterium,Methanoculleus,Methanocorpusculum,Methanosaeta and Methanospirillum.Diversity was obvious in the predominant archaeal from the pit mud with different cellar age.The predominant archaeal from the pit mud with cellar age of 20 years were Methanosarcina and Methanosaeta,which produced methane by utilizing acetic acid.However,the predominant archaeal from the pit mud with cellar age of 50 and 150 years were Methanocorpusculum and Methanobacterium,which utilizing hydrogen.With the increasing cellar age,the Shannon-Wiener index(H) and Berger-Parker index(D) of archaeal in the pit mud showed an increasing trend,while the Pielou index(J) showed a decreasing trend.The Sorenson similarity index of the pit mud with different cellar age was the range of 0.429~0.677,and there was the highest similarity of community structure between the pit mud with cellar age of 50 and 150 years.This study revealed the relationship between archaeal phylogenetic diversity of pit mud and the aging of pit mud.