In the present research, we discuss the results of analysis of coherent light beams carrying an optical vortex and propagating through the isotropic medium with a complex surface microrelief and its application to super resolution microscopy. It was shown, that phase analysis of singular beam with single charged centered optical vortex allow to retrieve information about sample surface relief. High spatial resolution caused by vortex helical phase sensitivity to disturbances in wave front after reflection or spreading through studying sample, which can be optically transparent or have a reflecting surface. This method applicable for non-destructive testing of live cells and biological tissues in real-time regime with exceeding optical diffraction limit. Vertical resolution of a microscope based on the phase singularity of Laguerre-Gaussian beams of low order can be achieved down to 5,27 nm for helium-neon laser source for optically transparent and reflecting surfaces.
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Sokolenko et al. (2018) studied this question.
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