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Fluorescein is widely used in ophthalmology for various purposes. Ehrlich (I882) observed that fluorescein introduced intravenously appeared in the aqueous humour. This phenomenon was employed for examination of the blood-aqueous barrier by Amsler (I946). Pfluger (I882) introduced a method of staining corneal erosion and ulceration by the use of fluorescein.
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Mitsui et al. (1969) studied this question.