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Abstract —A direct chemical method for the estimation of absolute amounts of synthetic melanin is established by the use of sodium borohydride, a mild reducing agent for the ketonic or aldehyde carbonyl group. Sodium borohydride not only solubilized the melanins obtained from various precursors, but it also restored aromatic characteristics in the absorption spectra. The solubilized melanin has also been used with a radio‐assay method for quantification of synthetic melanin. With this method of solubilization, the presence of lipids or proteins does not alter the linearity of the assay. The limiting amount of melanin detected by this method is 4‐5 μg/ml of the final solution.
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Das et al. (1976) studied this question.
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