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Protocol demonstrates optimized flash-freezing and single-nuclei suspension preparation from amniote inner-ear sensory epithelia, suggesting improved transcriptomic profiling for fragile tissues.
The inner-ear sensory epithelia are characteristically difficult to dissociate into healthy single-cell suspensions, with adult tissue presenting the most challenges. Recent advances in RNA sequencing have allowed for transcriptomic profiling of single nuclei, instead of single cells, offering a valuable alternative for processing sensitive tissue for single-cell genomics. Here, we provide a detailed protocol for flash-freezing, pooling, and preparing single nuclei suspensions from mouse and chicken inner-ear sensory epithelia, with a focus on optimization and quality control.
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Lloret et al. (2026) studied this question.
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