Comparative genomic study reveals distinct satellite repeat distributions in Triticeae species, expanding chromosomal markers for wheat breeding and introgression analysis.
Key Points
Identify and characterize the diversity, copy numbers, and chromosomal organization of satellite DNA repeats across Elymus and related Triticeae species.
Analyzed 14 Triticeae species using low-coverage whole-genome sequencing, graph-based repeat clustering, and qPCR.
Performed multivariate statistics (PCA, hierarchical clustering, Spearman correlation) and fluorescence in situ hybridization (FISH) karyotyping on wheat-Dasypyrum villosum lines.
Identified 16 satellite repeat clusters (E1–E16; monomer lengths 118–667 bp), separating perennial Elymus and Pseudoroegneria from Triticum, Secale, Hordeum, and Dasypyrum.
Mapped V-genome-specific repeats using FISH, localizing E6 to chromosome arms 3VL, 4VS, and 7VS, and E11 to 4VL.
Reclassified wheat-D. villosum lines W3 and W4 as 3V(3D) and 4V(4B) substitution lines, respectively, while confirming W7 as a 7V addition line.