What question did this study set out to answer?

To develop and validate a reproducible, controlled sheath inoculation protocol for evaluating rice varietal resistance to the fungal pathogen Cercospora janseana.

August 20, 2026Plant Disease

Development of a Sheath Inoculation Protocol to Screen Rice Varieties for Resistance to Cercospora janseana

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Authors

BBBernard O. Budot JSJacob SearightLouisiana State University Agricultural Center BABrijesh AngiraLouisiana State University Agricultural Center

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Implication

Controlled study reveals an agar disc sheath inoculation method reliably differentiates fungal resistance in rice varieties, providing a standardized platform for disease screening.

Key Points

To develop and validate a reproducible, controlled sheath inoculation protocol for evaluating rice varietal resistance to the fungal pathogen Cercospora janseana.
Evaluated three inoculation techniques (agar disc, spray, and drop) with and without mechanical wounding on rice sheaths.
Validated the optimized agar disc protocol across five independent trials assessing four rice varieties (DG263L, Cheniere, PVL03, and LaGrue) at 30-day and 45-day plant growth stages using lesion length and AUDPC metrics.
Symptom development strictly required mechanical wounding, with the agar disc method yielding the most uniform lesion expansion and lower variability than spray inoculation.
The protocol effectively separated resistance phenotypes: DG263L showed minimal lesion growth (resistant), Cheniere developed extensive lesions (highly susceptible), and PVL03 developed significantly greater lesion lengths and AUDPC values than LaGrue (moderately susceptible).

Cite This Study

Budot et al. (2026) studied this question.

synapsesocial.com/papers/6a86b5da8a91293e6a1cd552 https://doi.org/10.1094/pdis-02-26-0292-re

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Implication

Controlled study reveals an agar disc sheath inoculation method reliably differentiates fungal resistance in rice varieties, providing a standardized platform for disease screening.

Development of a Sheath Inoculation Protocol to Screen Rice Varieties for Resistance to Cercospora janseana

Discussion

Member takes

Implication

Key Points

Cite This Study

Development of a Sheath Inoculation Protocol to Screen Rice Varieties for Resistance to Cercospora janseana

Discussion

Member takes

Implication

Key Points

Cite This Study

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