Characterization of a region of the primary sequence of troponin C involved in calcium ion-dependent interaction with troponin I

R A Weeks; S V Perry

doi:10.1042/bj1730449

August 1, 1978Biochemical JournalOpen Access

Characterization of a region of the primary sequence of troponin C involved in calcium ion-dependent interaction with troponin I

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Key Points

To identify and characterize the specific peptide region within skeletal troponin C responsible for its calcium-dependent functional interactions with troponin I.
Digested rabbit fast skeletal muscle troponin C with cyanogen bromide (CNBr).
Isolated peptide fragments using gel filtration and affinity chromatography.
Assayed calcium-dependent complex formation with troponin I, neutralization of actomyosin Mg2+-ATPase inhibition, and inhibition of cAMP-dependent protein kinase phosphorylation.
Isolated peptide residues 83–134 formed a Ca2+-dependent complex with troponin I and fully neutralized troponin I inhibition of desensitized actomyosin Mg2+-ATPase.
Peptide 83–134 inhibited cyclic AMP-dependent protein kinase phosphorylation of fast skeletal muscle troponin I as effectively as intact troponin C on a molar basis, but had no effect on cardiac troponin I.
Chromatographic separation of the CNBr digest identified two distinct peptides, including fragment 83–134, that engaged in Ca2+-dependent binding with troponin I.

Structured PICO

P

Population

Troponin C from rabbit fast skeletal muscle

I

Intervention

CNBr digest of troponin C and isolated peptide (residues 83-134)

C

Comparator

Whole skeletal-muscle troponin C

O

Outcome

Calcium ion-dependent interaction with troponin I and neutralization of ATPase inhibitionsurrogate

Identifies a specific region (residues 83-134) of skeletal muscle troponin C responsible for calcium-dependent interaction with troponin I.

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Implication

No immediate clinical implications for muscle disorders; extends mapping of troponin C regulatory domains in animal models.

Abstract

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Cite This Study

Weeks et al. (1978) studied this question.

synapsesocial.com/papers/6a86c067e0e6abca40d3b677 https://doi.org/10.1042/bj1730449

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Characterization of a region of the primary sequence of troponin C involved in calcium ion-dependent interaction with troponin I

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Characterization of a region of the primary sequence of troponin C involved in calcium ion-dependent interaction with troponin I

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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