The sedimentation constant, diffusion constant and molecular weight of lactoglobulin

In a previous paper (Cecil & Ogston, 1948) we have described experiments directed to improving and testing the accuracy of the ultracentrifuge, using lactoglobulin as a test substance. We satisfied our- selves that we could measure sedimentation constants correct to 0-5 %. Lactoglobulin was used as a working substance in those experiments; we describe here some further experiments on its sedi- mentation and diffusion. EXPERIMENTAL Materials. Three preparations of lactoglobulin were made