The iron chelator desferrioxamine (DEF), commonly used to assess the involvement of iron in oxy-radical production, was examined with respect to its effect on spin trapping oxy-radicals generated independently of iron. OH. was generated by photolysis of H2O2- was generated by photolysis of Cds dispersions and by the hypoxanthine-xanthine oxidase system.In the presence of DEF, the amount of the radicals detected by spin trapping techniques of ESR with 5,5-dimethyl-1-pyrroline-1-oxide was greatly diminished. These results confirmed that DEF is an oxy-radical scavenger and, in addition, indicated that it should not be used to assess the involvement of iron in oxy-radical generation during spin trapping with nitrones for ESR analysis. An additional finding was that a new radical, as yet uncharacterized, was generated by UV irraditional of DEF.
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Hinojosa et al. (1986) studied this question.