In Vitro Study of DNA Interaction with Trichlorobenzenes by Spectroscopic and Voltammetric Techniques

In vitro studies on the interactions between native Herring Sperm DNA (HS-DNA) and 3 kinds of trichlorobenzenes (TCBs), i.e. 1,3,5-, 1,2,4-, 1,2,3-TCB, have been investigated by spectrophotometric, spectrofluorometric, melting temperature (Tm), viscosimetric and cyclic voltammetric techniques. The interaction constants between any of the TCBs and HS-DNA were found be at ~104 L.mol-1. The thermodynamic studies suggested that the interaction processes were endothermic disfavored (△H>0) and entropy favored (△S>0), which indicated that the TCBs might interact with HS-DNA by a non-traditional intercalation mode of binding via hydrophobic force.