October 1, 1976Infection and ImmunityOpen Access

Fate of rubella genome ribonucleic acid after immune and nonimmune virolysis in the presence of ribonuclease

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Population

Rubella virions containing radioactively labeled RNA

Comparison

Immune and nonimmune virolysis in the presence… vs Unlysed control pellets

Design

Preclinical

Authors

ASAnn SchluederbergNational Institute of Allergy and Infectious Diseases CAClayton AjelloHollister (United States)BEBarbara J. EvansMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

Member takes

Implication

Supports mechanistic studies of viral neutralization; does not alter current rubella prevention strategies.

Structured PICO

Population

Rubella virions containing radioactively labeled RNA

Intervention

Immune and nonimmune virolysis in the presence of ribonuclease (incubation with heat-inactivated rubella convalescent human serum and guinea pig complement, or unheated human immune sera, or unheated antibody-negative sera)

Comparator

Unlysed control pellets

Outcome

Accessibility of rubella virion RNA to nuclease attack (measured by increase in acid-soluble RNA, reduction in radioactivity, and sucrose gradient sedimentation profiles)surrogate

Rubella virion RNA becomes accessible to nuclease attack and is degraded after immune lysis of the viral envelope.

Cite This Study

Schluederberg et al. (1976) studied this question.

synapsesocial.com/papers/6a86d666d25f83eb47154855 https://doi.org/10.1128/iai.14.4.1097-1102.1976

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Fate of rubella genome ribonucleic acid after immune and nonimmune virolysis in the presence of ribonuclease

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Fate of rubella genome ribonucleic acid after immune and nonimmune virolysis in the presence of ribonuclease

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider