A rapid procedure has been developed for the isolation of methyl esters of trans ‐monoenoic fatty acids by silver ion high‐performance liquid chromatography. The combined saturated plus trans ‐monoene fraction was collected for analysis by gas chromatography and comparison with the composition of the unfractionated sample. The trans ‐monoene contents of partially hydrogenated vegetable oils, determined in this way, are compared with data obtained by Fourier‐transform infrared spectroscopy. Alternative methods of base‐line correction are discussed. The infrared technique gave values that were a little higher than the chromatographic ones, presumably because the former also detected trans unsaturation in di‐ and polyunsaturated fatty acids.
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Toschi et al. (1993) studied this question.
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