New antibiotic produced by Micromonospora globosa.

A new antibiotic, which we named deoxydynemicin A (1, Fig. 1), was produced together with dynemicin A1} (2, Fig. 1) in the culture broth of a strain of actinomycetes, MG331-hF6, which was isolated from a soil sample collected on Mt.Minobu, Yamanashi Prefecture, Japan.The strain was classified as Micromonospora globosa MG331-hF6 by taxonomic studies and deposited in the Fermentation Research Institute of the Agency of Industrial Science and Technology, Japan, with the accession No. FERM P-10651.Antibiotic 1 inhibited the growth of bacteria very strongly.Here, we report the production, isolation, properties, structure, and biological activities of 1 and NMR studies of 2.The stock culture of the strain MG331-hF6was cultured first in two 500-ml Erlenmeyer flasks each containing 100 ml of a medium consisting of glucose 1.0%, soybean flour (Prorich, Ajinomoto Co.) 2.0%, and dry yeast (Oriental Yeast Co.) 1.0%, at pH7.0 before sterilization.Incubation was on a rotary shaker at 30°C for 6 days.