What question did this study set out to answer?

To directly investigate whether artificial hydrostatic pressure differentials between adjacent plant cells induce the closure of plasmodesmata and stop intercellular transport.

September 1, 1992The Plant JournalOpen Access

Direct evidence for pressure‐generated closure of plasmodesmata

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Authors

KOKarl OparkaUniversity of Edinburgh DPD. A. M. PriorJames Hutton Institute

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Implication

Experimental study demonstrates that hydrostatic pressure differentials block intercellular transport in Nicotiana clevelandii trichomes, indicating turgor-sensitive plasmodesmata gating.

Key Points

To directly investigate whether artificial hydrostatic pressure differentials between adjacent plant cells induce the closure of plasmodesmata and stop intercellular transport.
Generated pressure differentials between adjacent leaf trichome cells of Nicotiana clevelandii using a modified micropressure probe and injection system.
Manipulated pressure via two methods: puncturing a cell to produce a differential with adjacent intact cells, or clamping cell pressure at high levels.
Assessed intercellular transport across cell walls by monitoring the basipetal movement of the fluorescent dye Lucifer yellow.
Intercellular transport of Lucifer yellow was blocked specifically at cell interfaces experiencing induced pressure gradients, such as the base of punctured cells and the top of clamped cells.
Pressure differentials exceeding 200 kPa were required to impede transport, whereas puncturing flaccid cells (<50 kPa) failed to block dye movement.
Cells clamped at elevated pressure partially retained transport inhibition after the probe was removed and turgor returned to normal.

Cite This Study

Oparka et al. (1992) studied this question.

synapsesocial.com/papers/6a86fd73a6ddb97d43d3c9a6 https://doi.org/10.1111/j.1365-313x.1992.tb00143.x

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Implication

Experimental study demonstrates that hydrostatic pressure differentials block intercellular transport in Nicotiana clevelandii trichomes, indicating turgor-sensitive plasmodesmata gating.

Direct evidence for pressure‐generated closure of plasmodesmata

Discussion

Member takes

Implication

Key Points

Cite This Study

Direct evidence for pressure‐generated closure of plasmodesmata

Discussion

Member takes

Implication

Key Points

Cite This Study

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