A sequential replication map of the chromosome of Bacillus licheniformis was constructed by employing the method of gene-frequency analysis presented by Yoshikawa and Sueoka. Our analysis of 11 genetic markers was based on the hypothesis that the chromosome initiated replication at a fixed origin and proceeded in a linear fashion to the terminus. The proposed locations of markers were validated by cotransformation and cotransduction analyses. Bacteriophage SP-15 cotransduced markers that failed to show linkage by transformation.
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Tyeryar et al. (1968) studied this question.