Lymphoid Cells Producing Antibody Against Simple Haptens: Detection and Enumeration

Enumeration of cells producing antibody against arsanilic acid was made feasible by the use of hapten-conjugated sheep erythrocytes for the localized hemolysis-in-gel technique. The time of appearance and numbers of these hapten-specific plaque-forming cells correlated closely with the development of arsanilic acid-specific serum antibodies.