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An accurate photometric method has been developed using a Pfalz and Bauer Fluorophotometer for measuring the lysis of suspensions of M. lysodiekticus by lysozyme. This method has the distinct advantages of reproducing activities in 60 seconds after addition of the enzyme. Measurement of maximal rate of lysis in the early part of the time-course of reaction decreases the possibility of interference due to synergism by proteolytic enzymes or solution of cellular material by alkali reaction. This method has been extended to extracts of mammalian tissues.
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Gerald Litwack (1955) studied this question.
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