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Rhodes' silver-plating technique for staining flagella was tested for its reliability and convenience as a routine procedure in the clinical laboratory. Modifications were made in the stain preparation and the procedure of staining and were tested with smears of known motile gram-negative nonfermentative bacilli. The stain has proved to be accurate and reliable and can be easily utilized with a minimum of training.
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West et al. (1977) studied this question.
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