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Details are given of a method for primary anaerobic cultivation in small plates of spirochetes from human and experimental fuso-spirochetal exudates, and for their pure subculture in plates and tubes. Successful primary plates were obtained in 23 instances out of 29 cases of human infection, and from all of 21 experimentally infected guinea pigs. Eleven strains of spirochetes have been isolated and subcultured in a short period with little difficulty.
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Rosebury et al. (1941) studied this question.
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