Perspectives on “Assessment of hormone measurement methods in girls with premature adrenarche, polycystic ovary syndrome, and non-classical congenital adrenal hyperplasia”

We read with interest the article by Uçar et al. entitled "Assessment of hormone measurement methods in girls with premature adrenarche, polycystic ovary syndrome, and non-classical congenital adrenal hyperplasia.". 1 Their comparison of traditional immunoassays with liquid chromatography -tandem mass spectrometry (LC-MS/MS) for measuring steroid hormones in hyperandrogenic conditions highlights an important diagnostic issue.Variability in outcomes of assay measurement can not only lead to unnecessary testing but may also delay diagnosis and affect outcomes.After carefully reviewing the methodology and results of the article, we would like to emphasize some points that may help to enhance the validity of the findings.