The present report describes a rapid, simple, and highly selective approach to perform testosterone competitive immunoassay by CE and LIF detection. The method involves using synthesized fluorescence-labeled testosterone as a tracer to compete with testosterone. Two polyclonal antibodies (antibody (Ab) arised from T-3-BSA (Ab(3)) and Ab arised from T-17-BSA (Ab(17))) and their respective tracers have been optimized and Ab(3) system is used for the quantification of testosterone by CE-based immunoassay. The method is developed with a wide working range of 3.70-2000 ng/mL and an LOD at 1.11 ng/mL. Tests for normal and positive urine samples show that this method has the potential to be applied in testosterone doping control.
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Chen et al. (2008) studied this question.
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