A high-performance gel permeation chromatographic (GPC) method was developed to determine the molecular weight distribution of pectins. The chromatographic system consisted of a hydrophilic coated silica (SynChropak) as the packing and a pH 3.7 acetate buffer as the mobile phase. By use of this system, high-methoxy, low-methoxy, and amidated pectins could be analyzed within fifteen minutes. By determining partition coefficients (Kd) of pectins as a function of mobile phase composition, Kd values were found to be independent of ionic strength from 0.055 to 0.7 M using pH 3.7 acetate buffers, which was in agreement, with intrinsic viscosity data.
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Howard G. Barth (1980) studied this question.
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