Methyl Groups as Probes of Structure and Dynamics in NMR Studies of High‐Molecular‐Weight Proteins

Solution NMR studies of biomolecules are typically limited by the molecules' molecular weight. Here we report strategies based on the use of highly deuterated, methyl‐protonated proteins that facilitate both structural and motional studies of molecules on the order of 100 kDa. Approaches and concepts are illustrated with applications to the enzyme malate synthase G, a monomeric 723‐residue protein.