Knockout of the IRF7 gene in HEK293FT cells alters their susceptibility to influenza A infection and affects the expression of interferon-regulated genes.
IRF7-knockout HEK293FT lines enable antiviral studies; leaves open cardiovascular relevance pending validation.
Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the factors that are quickly activated and are involved in a cellular response to a viral infection. In this work, monoclonal lines, based on HEK293FT cells defective in the IRF7 gene, were obtained using a CRISPR-Cas9 genome-editing method. These lines differed in the viability, proliferation rate, and susceptibility to infection with influenza A virus. Transcriptomic analysis of the most susceptible cell clone revealed differential expression of IRF7 factor as well as the other interferon-regulated genes.
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Komissarov et al. (2019) studied this question.
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