Locatelli et al. (6) reported on the quantitative detection of human herpesvirus 6 (HHV-6) in plasma and cell suspensions by TaqMan-based PCR. We wish to add our experience. We have developed a rapid (<30-min) assay for the simultaneous detection, quantification, and differentiation of HHV-6 variants A and B using a LightCycler (LC; Bio/Gene Ltd., Kimbolton, England). The instrument performs real-time PCR, combining rapid cycling with fluorescence-based identification of PCR products in glass reaction capillaries (1).
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Kearns et al. (2001) studied this question.
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