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Vacuoles containing S-adenosylmethionine have been isolated from the yeast, Candida utilis, by the osmotic lysis of its spheroplasts and differential centrifugation. Culture in the presence of L-methionine and harvest in the stationary phase contributed to the ease of isolation and stability of the vacuoles. The vacuoles showed retentiveness for the sulfonium compound and responded to the vital-staining reaction with Neutral Red. Ultraviolet micrography indicated the homogeneity of the vacuolar preparation.
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Kenji Nakamura (1973) studied this question.
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