Cultivation of Bacterium tularense in Peptone Media.

Summary1. Bacterium tularense grew luxuriantly and retained virulence during consecutive transfers in peptone broth and on peptone agar without blood or blood constituents. 2. Large inocula were required with the strains tested. The minimum effective inoculum was decreased by reducing agents or by a lowered oxygen tension over the culture, but growth was found to be dependent upon oxygen and stimulated by increased aeration. 3. Bacterium tularense appears to be an obligatory aerobe sensitive to mildly oxidizing environment.