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Amperometric biosensor based on the entrapment of polyphenol oxidase within a laponite clay coating and cross‐linked by glutaraldehyde is described for catechol detection. Laponite provides a hydrophilic enzyme surrounding increasing the long term stability of the biosensor compared to the corresponding biosensors obtained by chemical cross‐linking of PPO with glutaraldehyde. Azure B, a cationic dye exchanged within the clay matrix, is used as an electron shuttle allowing the mediated detection of phenol derivatives at −0.05 V. The detection limits obtained with the optimized biosensor configuration for catechol, p ‐cresol and phenol are 1, 1 and 17 nM, respectively.
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Shan et al. (2003) studied this question.
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