Our investigation of the analysis of vitamin D 3 metabolites has been reviewed. The development of high‐performance liquid chromatographic methods for the quantitative determination of 25‐hydroxyvitamin D 3 3‐sulfate and 25‐hydroxyvitamin D 3 , which are the major circulating metabolites of vitamin D 3 in human serum/plasma, has been described. The developed methods were applied to the determination of the correlation between the concentration of the sulfate and its genin in healthy subjects and patients with chronic renal failure. The development of immunoaffinity chromatography immobilizing the highly specific anti‐1,25‐dihydroxyvitamin D 3 antibody for the pretreatment of radioreceptor assay of 1,25‐dihydroxyvitamin D 3 , which is the active metabolite of vitamin D 3 , is also described.
No takes yet. Share an insight, caveat, or question.
Shimada et al. (2000) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: