Why the study?
Does centrifugation at 3,500 x g for 15 minutes maintain the sensitivity of detecting viruses in clinical specimens compared to 700 x g for 40 minutes?
Does centrifugation at 3,500 x g for 15 minutes maintain the sensitivity of detecting viruses in clinical specimens compared to 700 x g for 40 minutes?
Increasing centrifugation force to 3,500 x g for 15 minutes maintains viral detection sensitivity while reducing toxicity to the cell monolayer.
May enable shorter, higher-force shell vial protocols in labs; leaves open prospective validation before routine adoption.
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
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Engler et al. (1994) studied this question.
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