In a previous report, we detected the novel PSP-related protein in urine from a patient with diabetic nephropathy. This protein was different from PSP S1 and PSP S2-5 on the elution points of Mono S chromatography. To investigate the NH2-terminal sequence, we purified this protein by reverse phase chromatography and performed amino acid analysis. The results showed that the NH2-terminal sequence of this protein was one residue (arginine) longer than that of PSP S1.
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Kato et al. (1995) studied this question.
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