Evaluation of Memory Enhancing Potential of Centella lujica Supplement and Its Underlying Mechanisms of Action in mice model of Stress

Chronic stress is known to impair cognitive functions, particularly memory. This study investigated the neuroprotective and memory-enhancing effects of Centella lujica (CL) in a mouse model of chronic unpredictable mild stress (CUMS). Sixty adult male mice were divided into five groups: group 1 served as the control, groups 2 - 5 were exposed to CUMS, groups 3 and 4 were pretreated with CL at 25 mg/kg and 50 mg/kg orally, respectively, while group five was pretreated with donepezil (1 mg/kg, i.p.). Behavioral assessment using the novel object recognition test and biochemical analyses of the oxidative stress biomarkers, as well as the histology of the prefrontal cortex and hippocampus were done. Results showed that Centella lujica significantly enhanced memory performance (0.4120 ± 0.01715, 0.1920 ± 0.01281, 0.4240±0.02600, 0.4740±0.02293, and 0.3120±0.01985) and increased reduced glutathione levels (26.52±1.059, 17.8±1.499, 25.77±1.636, 29.7±1.535, and 24.82±1.631 in the prefrontal cortex; 18.8±0.6092, 13.21±0.6095, 18.38±0.773, 19.78±0.6057, and 17.31±0.5267 in the hippocampus), indicating the potential of Centella lujica in reducing oxidative stress. Histological evaluation confirmed improved structural integrity in the prefrontal cortex and hippocampus. These findings suggest that Centella lujica counteracts stress-induced oxidative damage and promotes neuroplasticity, offering potential therapeutic benefits for stress-related cognitive impairments. Further studies are needed to explore its clinical applications.