Our laboratory recently recovered Chlamydia trachomatis in tissue culture from a urogenital specimen which tested negative by commercial plasmid-based PCR. Immunotyping and omp1 sequencing identified the isolate as a serovar E isolate. Further investigation by PCR and Southern hybridization indicated that this isolate lacks the chlamydial cryptic plasmid.
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Stothard et al. (1998) studied this question.
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