December 14, 2012PLoS ONEOpen Access

Use of the Foot-and-Mouth Disease Virus 2A Peptide Co-Expression System to Study Intracellular Protein Trafficking in Arabidopsis

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Population

Arabidopsis plant cells (protoplast and in planta)

Design

Preclinical

Authors

SBStefan BurénConsejo Superior de Investigaciones Científicas COCristina Ortega‐VillasanteUniversidad Autónoma de Madrid KÖKrisztina ÖtvösInstitute of Science and Technology Austria

Discussion

Member takes

Implication

Supports 2A peptide for plant trafficking studies; leaves open validation in stable systems or other species.

Structured PICO

Population

Arabidopsis plant cells (protoplast and in planta)

Intervention

Co-expression of dominant mutant forms of GTPases (RABD2a, SAR1, ARF1) with fluorescent marker proteins using the 2A peptide system

Outcome

Cleavage efficiency of the 2A peptide and inhibitory effect on trafficking of CAH1 proteinsurrogate

The 2A peptide system is an effective tool for studying intracellular protein trafficking in plant cells, revealing that CAH1 trafficking depends on canonical vesicular transport between the ER and Golgi.

Cite This Study

Burén et al. (2012) studied this question.

synapsesocial.com/papers/6a8813362c11edba2ecf0d53 https://doi.org/10.1371/journal.pone.0051973

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Use of the Foot-and-Mouth Disease Virus 2A Peptide Co-Expression System to Study Intracellular Protein Trafficking in Arabidopsis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Use of the Foot-and-Mouth Disease Virus 2A Peptide Co-Expression System to Study Intracellular Protein Trafficking in Arabidopsis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider