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Objective : We studied the relationship between the severity of inflammation and IL-1 β production and relative expression level of IL-1 β mRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis. Methods : Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1 β protein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1 β mRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes. Results : IL-1 β concentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1 β mRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity. Conclusions : IL-1 β concentration in irrigation fluid and relative expression level of IL-1 β mRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.
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Chia et al. (2009) studied this question.
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