Key result
Virus screening in indigenous Greek black pigs detected PCMV/PRV, PLHV-1, PLHV-3, PVC3, and PERV-C in most animals, whereas PPV1, HEV3, PCV4, and PERV-A/C were not detected.
Why the study?
The study was conducted to determine the sensitivities of detection methods for multiple potentially zoonotic porcine viruses and to apply these methods to screen indigenous Greek black pigs.
Observational
Screening of indigenous Greek black pigs validated highly sensitive viral detection methods and identified the presence of several viruses, which is relevant for ensuring the safety of future xenotransplantation.
Emphasizes viral screening needs in porcine xenotransplant donors; leaves open clinical translation from animal models.
The successful advancement of xenotransplantation has led to the development of highly sensitive detection systems for the screening of potentially zoonotic viruses in donor pigs and preventing their transmission to the recipient. To validate these methods, genetically modified pigs generated for xenotransplantation, numerous minipigs and other pig breeds have been tested, thereby increasing our knowledge concerning the pig virome and the distribution of pig viruses. Of particular importance are the porcine cytomegalovirus, a porcine roseolovirus (PCMV/PRV) and the hepatitis E virus genotype 3 (HEV3). PCMV/PRV has been shown to reduce the survival time of pig transplants in non-human primates and was also transmitted in the first pig heart transplantation to a human patient. The main aim of this study was to determine the sensitivities of our methods to detect PCMV/PRV, HEV3, porcine lymphotropic herpesvirus-1 (PLHV-1), PLHV-2, PLHV-3, porcine circovirus 2 (PCV2), PCV3, PCV4 and porcine parvovirus 1 (PPV1) and to apply the methods to screen indigenous Greek black pigs. The high number of viruses found in these animals allowed for the evaluation of numerous detection methods. Since porcine endogenous retroviruses (PERVs) type A and B are integrated in the genome of all pigs, but PERV-C is not, the animals were screened for PERV-C and PERV-A/C. Our detection methods were sensitive and detected PCMV/PRV, PLHV-1, PLHV-1, PLHV-3, PVC3 and PERV-C in most animals. PPV1, HEV3, PCV4 and PERV-A/C were not detected. These data are of great interest since the animals are healthy and resistant to diseases.
No takes yet. Share an insight, caveat, or question.
Jhelum et al. (2024) conducted an observational in Zoonotic viruses in pigs. Virus screening was evaluated on Detection of zoonotic viruses including PCMV/PRV, PLHV, PCV, PPV1, HEV3, and PERV. Virus screening in indigenous Greek black pigs detected PCMV/PRV, PLHV-1, PLHV-3, PVC3, and PERV-C in most animals, whereas PPV1, HEV3, PCV4, and PERV-A/C were not detected.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: