Key result
A novel sandwich ELISA was developed to quantify human soluble (pro)renin receptor, demonstrating high linearity (125–8,000 pg/ml) and detecting mean plasma levels of 22.31 ng/ml in healthy volunteers.
The newly developed sandwich ELISA provides a reliable and precise method for quantifying soluble (pro)renin receptor levels in human plasma, serum, and urine.
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Enables sPRR measurement in human plasma, serum, and urine; leaves open its clinical or prognostic utility pending validation studies.
The (pro)renin receptor ((P)RR) plays a key role in the activation of the local renin-angiotensin system via interaction with renin and prorenin. A truncated form that is cleaved by furin, referred to as soluble (pro)renin receptor (s(P)RR), is secreted into the extracellular space. An accurate measurement of s(P)RR levels in vivo is an important issue in elucidating the roles of (P)RR in physiology and pathophysiology. To address this, we developed a sandwich ELISA that is applicable to human subjects. The standard curve of this ELISA showed a high linearity (125-8,000 pg/ml) with a correlation coefficient of >0.99. The recovery rate was approximately 90% in human blood and urine samples. The s(P)RR levels in plasma of healthy volunteers was in the range from 15.2 to 35.1 ng/ml (n = 20). Intra- and inter-assay coefficient of variations were less than 5.5% and 7.5%, respectively. It thus appears that this ELISA is a reliable tool for measuring s(P)RR levels in human subjects.
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Nobuhiro Maruyama (2012) studied Healthy (n=20). Novel sandwich ELISA for s(P)RR was evaluated on s(P)RR levels in plasma of healthy volunteers. A novel sandwich ELISA was developed to quantify human soluble (pro)renin receptor, demonstrating high linearity (125–8,000 pg/ml) and detecting mean plasma levels of 22.31 ng/ml in healthy volunteers.
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