Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells.

OBJECTIVE: in human hepatoma HepG2 cells. MATERIALS AND METHODS: at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by MTT assay. The apoptosis rate was investigated by Hoechst 33342 and annexin V/propidium iodide staining. Mitochondrial membrane potential was evaluated by rhodamine staining. Also, the expression of catenin - β 1 protein was analyzed by western blotting. RESULTS: treatments. This treatment also modulated the catenin - β 1 protein expression. CONCLUSION: in HepG2 cells; thus, this plant may be beneficial in the treatment of liver cancer.