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This unit contains protocols for the use of lactose‐derived autoinduction in Escherichia coli . The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X‐ray structural studies, and multi‐well plate growth for screening and optimization are also included. Curr. Protoc. Protein Sci . 56:5.23.1‐5.23.18.
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Fox et al. (2009) studied this question.
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