The pH values of commonly-employed algal media respond markedly to the stream of CO2-in-air with which such media are frequently bubbled during the culturing of algae. This change in pH can be obviated by: (a) using only 1%CO2-in-air rather than the unnecessarily higher concentrations often used, and (b) by incorporating 0.05 MSigma 7–9 buffer (tris hydroxymethyl amino-methane) in the medium.
No takes yet. Share an insight, caveat, or question.
Galloway et al. (1961) studied this question.